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Molecular Characterization of an Ependymin Precursor from Goldfish Brain
Author(s) -
Königstorfer A.,
Sterrer S.,
Eckerskorn C.,
Lottspeich F.,
Schmidt R.,
Hoffmann W.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb10932.x
Subject(s) - neuroscience , characterization (materials science) , chemistry , biology , computational biology , nanotechnology , materials science
Ependymins are thought to be implicated in fundamental processes involved in plasticity of the goldfish CNS. Gas‐phase sequencing of purified ependymins β and γ revealed that they share the same N‐terminal sequence. Each sequence displays microheterogeneities at several positions. Based on the protein sequences obtained, we constructed synthetic oligonucleotides and used them as hybridization probes for screening cDNA libraries of goldfish brain. In this article we describe the full‐length sequence of a mRNA encoding a precursor of ependymins. A cleavable signal sequence characteristic of secretory proteins is located at the N‐terminal end, followed directly by the ependymin sequence. Also, two potential N‐glycosylation sites were detected. A computer search revealed that ependymins form a novel family of unique proteins.