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Carbachol‐Induced Cosecretion of Immunoreactive Atrial Natriuretic Peptides with Catecholamines from Cultured Bovine Adrenal Medullary Cells
Author(s) -
Okazaki Masahiro,
Yanagihara Nobuyuki,
Izumi Futoshi,
Nakashima Yasuhide,
Kuroiwa Akio
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb10920.x
Subject(s) - medicine , endocrinology , adrenal medulla , atrial natriuretic peptide , carbachol , nicotinic agonist , muscarinic acetylcholine receptor , chemistry , chromaffin cell , catecholamine , receptor , stimulation , biology
The distribution and secretion of atrial natriuretic peptides (ANPs) were investigated in bovine adrenal medulla. (1) Cultured bovine adrenal medullary cells (2 × 10 6 /dish) contained 100.4 ± 6.0 fmol of immunoreactive ANP (IR‐ANP) and 207.3 ± 6.6 nmol of catecholamines as epinephrine plus norepinephrine. (2) Stimulation of nicotinic but not muscarinic acetylcholine receptors caused a cosecretion of IR‐ANP and catecholamines corresponding to the ratio of IR‐ANP to catecholamines in cultured bovine adrenal medullary cells. (3) Carbachol‐stimulated secretion of IR‐ANP was dependent on the presence of extracellular Ca 2+ . (4) Chromaffin granules isolated from bovine adrenal medulla contained large amounts of IR‐ANP and catecholamines, in the same ratio as did cultured adrenal medullary cells. (5) Reverse‐phase HPLC analysis showed that both stored and secreted IR‐ANP consisted of two components, which eluted at the position of ANP(99–126) or ANP(1–126). These results indicate that ANPs are stored as ANP(99–126) and ANP(1–126) in chromaffin granules, and are cosecreted in parallel with catecholamines in a Ca 2+ ‐dependent manner by the stimulation of nicotinic acetylcholine receptors.