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Nerve Growth Factor Potentiates the Hormone‐Stimulated Intracellular Accumulation of Inositol Phosphates and Ca 2+ in Rat PC12 Pheochromocytoma Cells: Comparison with the Effect of Epidermal Growth Factor
Author(s) -
Calker Dietrich,
Takahata Kyoya,
Heumann Rolf
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb10895.x
Subject(s) - epidermal growth factor , endocrinology , medicine , inositol , nerve growth factor , second messenger system , intracellular , inositol phosphate , cytosol , carbachol , inositol trisphosphate , growth factor , biology , chemistry , receptor , microbiology and biotechnology , biochemistry , stimulation , enzyme
The effects of nerve growth factor (NGF) and epidermal growth factor (EGF) on the intracellular accumulation of inositol phosphates and on cytosolic free Ca 2+ concentrations were studied in rat PC12 pheochromocytoma cells. Both NGF and EGF potentiate in these cells the increase in the accumulation of inositol phosphates that is elicited by bradykinin and carbachol. A corresponding potentiation was also found for the agonist‐induced increase of cytosolic Ca 2+ concentrations. The effect of NGF, but not that of EGF, is abolished when the cells are preincubated with 5′‐deoxy‐5′‐methylthioadenosine, an inhibitor of S ‐adenosylhomocysteine hydrolase. These results suggest that an increased response to hormones, which act via phosphoinositide‐derived second messengers, may be important in the mechanism of action of NGF and EGF.