Premium
Does the Olfactory System Mediate Water‐ and Mineral‐Regulating Mechanisms? Evidence of Immunoreactive Atrial Natriuretic Factor Within Olfactory Mucosa
Author(s) -
Gutkowska Jolanta,
Marcinkiewicz Mieczyslaw
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09249.x
Subject(s) - autocrine signalling , medicine , endocrinology , paracrine signalling , endogeny , olfactory mucosa , immunocytochemistry , biology , receptor , kidney , chemistry , peptide hormone , olfactory system , hormone , biochemistry , neuroscience
The immunoreactivity of atrial natriuretic factor (ANF) was studied in the rat olfactory mucosa (OM). Endogenous immunoreactive ANF (IR‐ANF) was purified from OM using Vycor glass beads for extraction and reverse‐phase HPLC: two of three IR‐ANF peaks, identified by retention time, were identical to both the circulating form of ANF (Ser 99 ‐Tyr 126 ) and the ANF pro‐hormone (Asn 1 ‐Tyr 126 ). A radioreceptor assay, employing rat renal glomerular membranes, revealed that endogenous IR‐ANF competed with radiolabeled ANF. IR‐ANF was localized by immunocyto‐chemistry in secretory cells of Bowman's gland and in some cells of the epithelial layer. The relatively low concentration of IR‐ANF in the OM (2.5 ng/mg protein) suggests a local role of ANF in this tissue. This hypothesis is supported by the presence in OM of ANF‐binding sites, characterized by a K D of 95 p M and a B max of 130 fmol/mg protein. We propose that ANF could be released from the OM and act throughout in a paracrine (if not autocrine) manner on some yet‐unidentified targets containing ANF‐binding sites.