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Atrial Natriuretic Peptide Modulates Amiloride‐Sensitive Na + Transport Across the Blood‐Brain Barrier
Author(s) -
Ibaragi Masaaki,
Niwa Masami,
Ozaki Masayori
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09246.x
Subject(s) - amiloride , blood–brain barrier , brain natriuretic peptide , chemistry , atrial natriuretic peptide , medicine , peptide , biophysics , endocrinology , central nervous system , sodium , biochemistry , biology , heart failure , organic chemistry
We obtained evidence that amiloride specifically potentiates 125 I‐labeled α ‐rat atrial natriuretic peptide (1‐28) [atrial natriuretic peptide (ANP)‐(99‐126); rANP] binding to cerebral capillaries isolated from the rat cerebral cortex. The binding parameters, K D of 173 p M and B max of 159 fmol/ mg of protein, became 33 p M and 88 fmol/mg of protein, respectively, when 10 −4 M amiloride was added to the incubation medium. When the effect of rANP was investigated on in vitro 22 Na + uptake into isolated cerebral capillaries, 10 −7 M rANP significantly inhibited the uptake in the presence of 1.0 m M ouabain, 1.0 m M furosemide, and 2.0 m M LiCI in the uptake buffer, a finding suggesting a specific inhibitory effect of rANP on amiloride‐sensitive Na + transport. Thus, the possibility that ANPs control amiloride‐sensitive Na 4+ transport at the blood‐brain barrier by interacting with specific receptors has to be considered