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Analysis of Biogenic Amines in Bovine Retina by Gas Chromatography‐Negative Ion Chemical Ionisation Mass Spectrometry
Author(s) -
Macfarlane R. G.,
Macleod S. C.,
Midgley J. M.,
Watson D. G.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09238.x
Subject(s) - chemistry , mass spectrometry , trimethylsilyl , chromatography , chemical ionization , gas chromatography , gas chromatography–mass spectrometry , extraction (chemistry) , hydrolysis , chloride , ion , thermospray , detection limit , solvent , organic chemistry , ionization , selected reaction monitoring , tandem mass spectrometry
Biogenic amines in bovine retina have been identified and quantified by an extraction‐derivatisation procedure involving their reaction with 3,5‐di(trifluoromethyl)benzoyl chloride (DTFMBCl) in the aqueous phase followed by extraction into an organic solvent, hydrolysis of phenolic esters, and conversion of free hydroxyl groups to trimethylsilyl ethers. Subsequent analysis of these DTFMB‐trimethylsilyl derivatives by gas chromatography‐negative ion chemical ionisation mass spectrometry revealed that the molecular ion carried most (>60%) of the ion current, which made the method highly specific and gave a potential limit of detection below the picogram level. This method establishes unequivocally that the principal amines in bovine retina are p ‐tyra‐mine, dopamine, and 5‐hydroxytryptamine