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Calcium Uptake and Protein Phosphorylation in Myenteric Neurons, Like the Release of Vasoactive Intestinal Polypeptide and Acetylcholine, Are Frequency Dependent
Author(s) -
Agoston Denes V.,
Lisziewicz Julianna
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09219.x
Subject(s) - vasoactive intestinal peptide , acetylcholine , nifedipine , stimulation , endocrinology , medicine , calcium , chemistry , neurotransmitter , voltage dependent calcium channel , biology , neuropeptide , receptor , central nervous system
The mechanism of the electrical‐to‐chemical decoding involved in the preferential release of the transmitters acetylcholine and vasoactive intestinal polypeptide (VIP) by electrical field stimulation at low (5 Hz) and high (50 Hz) frequencies was studied in superfused myenteric neurons. The stimulation‐induced uptake of 45 Ca 2+ accompanying high frequency stimulation was markedly reduced by 10 μ M nifedipine, a specific blocker of l ‐type voltage‐sensitive Ca 2+ channels (VSCCs), as was also the preferential high‐frequency release of VIP. By contrast, the 45 Ca 2+ uptake during low‐frequency stimulation was somewhat lower per pulse, and neither this uptake nor the preferential release of acetylcholine occurring at this frequency was significantly reduced by nifedipine. These findings suggest that the release of acetylcholine and VIP involve different VSCCs. The pattern of in vitro protein thiophosphorylation in tissue extracts of differentially stimulated myenteric neurons involved polypeptides of 205, 173, 86, 73, 57, 54, 46, 32, 28, and 24 kDa and was also markedly stimulus and nifedipine dependent. This suggests that different phosphoproteins are involved during the frequency‐dependent activation of the different Ca 2+ channels and exocytotic mechanisms.