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1‐Methyl‐4‐Phenylpyridinium Uptake by Human and Rat Striatal Synaptosomes
Author(s) -
Willoughby J.,
Cowburn R. F.,
Hardy J. A.,
Glover Vivette,
Sandler M.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09165.x
Subject(s) - chemistry , neuroscience , pharmacology , biophysics , biology
1‐Methyl‐4‐phenylpyridinium (MPP + ) was taken up into human and rat striatal synaptosomes by a saturable system, similar to that for dopamine, with K m values of 0.24 and 0.17 μ M , respectively, and similar V max values. Uptake of MPP + and dopamine into both rat and human synaptosomes was inhibited by cocaine and amfonelic acid, with the latter being five to 10 times more potent than the former. MPP + uptake was potently inhibited by dopamine in preparations from both species. In general, the characteristics of human and rat synaptosomal MPP + uptake were very similar. It seems unlikely that species differences in toxicity to 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine or reaction to dopamine uptake blockers stem from this system.