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Creatine Transport in Cultured Cells of Rat and Mouse Brain
Author(s) -
Möller Andreas,
Hamprecht Bernd
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09154.x
Subject(s) - creatine , neuron , chemistry , transport system , biology , medicine , endocrinology , biochemistry , neuroscience , transport engineering , engineering
Astroglia‐rich cultures derived from brains of newborn rats or mice use a transport system for the uptake of creatine. The uptake system is saturable, Na + ‐dependent, and highly specific for creatine and Na + . Kinetic studies on rat cells revealed a K m value for creatine of 45 μ M , a V max of 17 nmol X h −1 X (mg of protein) −1 , and a K m value of 55 m M for Na + . The carrier is competitively inhibited by guanidinopropionate ( K i = 15 μ M ). No such transport system was found in neuron‐rich primary cultures from embryonic rat brain. It is hypothesized that creatine transport is an astroglial rather than a neuronal function.