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K ‐Opiate Agonists Inhibit Adenylate Cyclase and Produce Heterologous Desensitization in Rat Spinal Cord
Author(s) -
Attali Bernard,
Saya Danielle,
Vogel Zvi
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb09130.x
Subject(s) - medicine , endocrinology , agonist , forskolin , chemistry , cyclase , desensitization (medicine) , pharmacology , receptor , biology , stimulation
The nature of the opiate modulation of adenylate cyclase following acute and chronic agonist exposure has been investigated in rat spinal cord. Using membranes of both adult rat spinal cord and spinal cord‐dorsal root ganglion cocultures, we found that K ‐opiate receptors are negatively coupled to adenylate cyclase. The K ‐opiate agonists (e.g., U50488) inhibit significantly and dose‐dependently the basal and the forskolin‐stimulated cyclase activities, whereas μ and δ agonists are ineffective. The regulatory action is stereospecific and requires the presence of GTP. EGTA treatment of the plasma membranes abolished the effect of K ‐opiate agonists on the basal cyclase activity, and this inhibitory effect could not be restored by subsequent addition of Ca 2+ . The EGTA treatment did not affect the K agonist inhibition of the forolin‐stimulated cyclase. The results also show that following chronic exposure of cultured cells to etorphine or U50488, there is a loss of K agonist inhibition of the cyclase. Moreover, this desensitization process appears to be heterologous, because α 2 ‐adrenergic agonists (e.g., clonidine or norepinephrine) and the muscarinic agonist (carbachol) exhibited significantly lower potency for inhibiting cyclase activity when compared to untreated cultures. This pattern of heterologous desensitization suggests that chronic exposure to K opiates leads to alterations in postreceptor regulatory components, possibly GTP‐binding proteins.