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Regulation of the γ‐Aminobutyric Acid A Receptor by γ‐Aminobutyric Acid Levels Within the Postsynaptic Cell
Author(s) -
Wood J. D.,
Davies M.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb08565.x
Subject(s) - postsynaptic potential , aminobutyric acid , muscimol , isonicotinic acid , organelle , chemistry , gamma aminobutyric acid , biochemistry , glutamate receptor , gabaa receptor , microbiology and biotechnology , biophysics , receptor , biology , hydrazide , organic chemistry
Synaptosomes and synaptoneurosomes were prepared from rat cerebral cortex. Comparison of the amino acid levels in the two types of organelles and of the effects of gabaculine thereon indicated that the neurosome portion of synaptoneurosomes constituted the major influencing component of the organelles. Administration to rats of inhibitors of γ‐aminobutyric acid (GABA) degradation, such as gabaculine and L‐cycloserine, resulted in elevated GABA levels in synaptoneurosomes and a decrease in muscimol‐stimulated C1 uptake by the organelles. Addition of gabaculine directly to the incubation medium for the uptake assay had no effect on the C1 transport. In contrast, administration to rats of isonicotinic acid hydrazide, an inhibitor of GABA synthesis, decreased the GABA level in synaptoneurosomes and increased the muscimol‐stimulated Cl − uptake by the organelles. Although the evidence is not unequivocal, it does support the concept of GABA released from nerve endings being taken up by the postsynaptic cell, from where it exerts a regulatory influence on the functioning of the GABA receptor/ion channel complex.