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Late‐Phase Accumulation of Inositol Phosphates Stimulated by Prostaglandins D 2 and F 2α in Neuroblastoma × Glioma Hybrid NG108‐15 Cells
Author(s) -
Hatanaka Michiyo,
Yumoto Noboru,
Miwa Naoto,
Morii Hiroshi,
Tanemura Mihoko,
Ueno Ryuji,
Watanabe Yasuyoshi,
Hayaishi Osamu
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb08537.x
Subject(s) - inositol , glioma , inositol phosphate , neuroblastoma , phase (matter) , chemistry , endocrinology , medicine , cancer research , biology , biochemistry , genetics , cell culture , receptor , organic chemistry
The accumulation of inositol phosphates (IPs) in response to prostaglandins (PGs) was studied in NG108‐15 cells preincubated with myo ‐[ 3 H]inositol. As a positive control, bradykinin caused accumulation of IPs transiently at an early phase (within 1 min) and continuously during a late phase (15‐60 min) of incubation in the cells. PGD 2 and PGF 2a did not significantly cause the accumulation of IPs at an early phase but significantly stimulated inositol bisphosphate (IP 2 ) and inositol monophosphate (IP 1 ) formation at a late phase of incubation. The maximum stimulation was obtained at <10 −7 M concentrations of these PGs. the levels being three‐and twofold for IP 2 and IP 1 respectively. 9α, 11 β‐PGF 2 has a slight effect but PGE 2 and the metabolites of PGD 2 and PGF 2α have no effect up to 10 −6 M . The effects of PGD 2 and PGF 2α were not additive, but the effect of each PG was additive to that of bradykinin at a late phase of incubation. Inositol 1‐monophosphate was mainly identified in the stimulation by 10 −5 M PGD 2 and 10 −5 MPGF 2a , whereas both inositol 1‐monophosphate and inositol 4‐monophosphate were produced in the stimulation by 10 −5 M bradykinin. Depletion of extracellular Ca 2+ diminished the stimulatory effect of PGD 2 and PGF 2α and late‐phase effect of bradykinin, but simple Ca 2+ influx into the cells by high K + , ionomycin, or A23187 failed to cause such late‐phase effects. These results suggest that PGD 2 and PGF 2α specifically stimulate hydrolysis of inositol phospholipids.