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Epidermal Growth Factor Enhances the Expression of an Endogenous Lectin in Aggregating Fetal Brain Cell Cultures
Author(s) -
Tenot Michè,
Kuchler Sabine,
Zanetta JeanPierre,
Vincendon Guy,
Honegger Paul
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb08535.x
Subject(s) - lectin , endogeny , biology , epidermal growth factor , mannan binding lectin , extracellular , microbiology and biotechnology , mannose , intracellular , c type lectin , cell culture , fetus , cerebrum , biochemistry , central nervous system , neuroscience , receptor , pregnancy , genetics
Aggregating cell cultures prepared from fetal rat telencephalon express the two subunits [cerebellar soluble lectins (CSL) 1 and 2] of a soluble, mannose‐specific endogenous lectin (CSL) in a development‐dependent manner. Increased CSL synthesis was found at an early postmitotic stage as well as during the period of maximal myelination. Repet‐tive treatment of early cultures with epidermal growth factor EGF, 3 n M ) caused a great stimulation of CSL biosynthesis. Immunocytochemical studies revealed particularly intense CSL‐specific staining in small, EGF‐responsive cells, presumably glial cells. Large quantities of CSL‐immunoreactive material were found also in the extracellular space and on the external side of the plasma membrane, indicating abundant release of CSL. The present findings suggest that EGF or EGF‐related factors in the brain are able to regulate the expression of an endogenous lectin, affecting brain ontogeny.