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Activation of Guanylate Cyclase by Bradykinin in Rat Sensory Neurones Is Mediated by Calcium Influx: Possible Role of the Increase in Cyclic GMP
Author(s) -
Burgess G. M.,
Mullaney I.,
McNeill M.,
Coote P. R.,
Minhas A.,
Wood J. N.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb07417.x
Subject(s) - bradykinin , sensory system , cyclic gmp , guanylate cyclase , calcium , chemistry , neuroscience , endocrinology , medicine , microbiology and biotechnology , biology , biochemistry , enzyme , receptor , organic chemistry
Bradykinin, which activates polymodal nociceptors, increased cyclic GMP (cGMP) in a capsaiciiji‐sensitive population of cultured sensory neurones from rat dorsal root ganglia (DRG) by stimulating guanylate cyclase, but had no effect on cyclic AMP (cAMP). In nonneunbnal cells from DRG, bradykinin increased cAMP, but not cpMP. The bra‐dykinin‐induced increase in cGMP in thej neurones was completely blocked by removal of extracellular Ca 2+ , or by incubation of the cells with the calcium channel blockers nifedipine and verapamil. Pretreatment of the| neurones with either dibutyryl cGMP or sodium nitroprusside (which elevates cGMP) inhibited bradykinin‐induced formation of inositol phosphates. It is possible that cGMP could be involved in the regulation of polyphosphoinositide turnover in DRG neurones.