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Changes in Intracellular Free Magnesium During Hypoglycaemia and Hypoxia in Cerebral Tissue as Calculated from 31p ‐Nuclear Magnetic Resonance Spectra
Author(s) -
Brooks K. J.,
Bachelard H. S.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb07338.x
Subject(s) - magnesium , intracellular , hypoxia (environmental) , chemistry , nuclear magnetic resonance , nucleoside triphosphate , nucleoside , spectral line , calcium , intracellular ph , magnetic resonance imaging , endocrinology , medicine , biophysics , oxygen , biochemistry , nucleotide , biology , physics , organic chemistry , radiology , astronomy , gene
3l P‐nuclear magnetic resonance spectra of super‐fused cerebral tissues were obtained under normal, hypogly‐caemic, and hypoxic conditions. Concentrations of free intracellular magnesium were calculated from differences in chemical shifts between the α‐ and β‐resonances of the nucleoside phosphates. Control levels of 0.33 m M were significantly increased to 0.52 m M in hypoglycaemia and to 0.57 m M in severe hypoxia. Removal of calcium from the superfusing medium increased the free intracellular Mg 2+ concentration to 0.63 m M