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p ‐Chloromercuribenzoate Causes Ca 2+ ‐Dependent Exocytotic Catecholamine Secretion from Cultured Bovine Adrenal Medullary Cells
Author(s) -
Tachikawa Eiichi,
Takahashi Saburo,
Kashimoto Takeshi
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb07290.x
Subject(s) - secretion , dithiothreitol , catecholamine , extracellular , intracellular , chemistry , exocytosis , medicine , chromaffin cell , endocrinology , adrenal medulla , biochemistry , enzyme , biology
Incubation of cultured bovine adrenal medullary cells with p ‐chloromercuribenzoate (50–500 μ M ), a sulfhy‐dryl‐reacting agent, caused an increase in the secretion of catecholamines. p ‐Chloromercuriphenyl sulfonate, a p ‐chlo‐romercuribenzoate analogue that poorly penetrates the cell membrane, caused a similar increase in catecholamine secretion. In both cases, catecholamine secretion was dependent on extracellular Ca 2+ . Furthermore, p ‐chloromercuribenzoate caused both 45 Ca 2+ influx into the cells and an increase in the intracellular free Ca 2+ concentration. The increases in catecholamine secretion and 45 Ca 2+ influx behaved similarly in relation to p‐chloromercuribenzoate concentration. The time courses of the increased secretion, 45 Ca 2+ influx, and intracellular free Ca 2+ concentration by p ‐chloromercuri‐benzoate were also quite similar. The stimulation of cate‐holamine secretion by p ‐chloromercuribenzoate was reversed by washing the cells with dithiothreitol‐containing medium, but not by dithiothreitol‐free medium. When the cells were treated with p ‐chloromercuribenzoate, dopamineβ‐hydroxylase, an enzyme present in the chromaffin granules along with catecholamines, was also released. However, p ‐chloromercuribenzoate did not cause release of phenyletha‐nolamine‐JV‐methyltransferase, an enzyme present in the cytoplasm. These results indicate that catecholamine secretion due to p ‐chloromercuribenzoate occurs by Ca 2+ ‐dependent exocytosis.