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Possible Involvement of Polysialogangliosides in Nerve Sprouting and Cell Contact Formation: An Ultracytochemical In Vitro Study
Author(s) -
Seybold Volker,
Rösner Harald,
Greis Christian,
Beck Eva,
Rahmann Hinrich
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb07286.x
Subject(s) - microbiology and biotechnology , ultrastructure , cell , sprouting , extracellular matrix , cell adhesion , epitope , biology , cell adhesion molecule , extracellular , neural cell , monoclonal antibody , chemistry , antibody , biophysics , anatomy , immunology , biochemistry , botany
In Cichlid fish ( Oreochromis mossambicus ) primary cell cultures from whole brain and optic tectum, the differentiation‐dependent distribution of polysialogangliosides on the outer cell surface has been followed on an ultrastructural level. For this, a two‐step labeling technique with the monoclonal mouse antibody Q211, recognizing a polysialoganglioside‐associated epitope, followed by a secondary IgM antibody, coupled to colloidal gold sols as an electron‐dense marker, has been used. The gold grains are not uniformly distributed over the whole cell surface, but rather are clearly arranged clusters. In cells from freshly hatched larvae, both cell bodies and nerve fibers strongly exhibit the polysialoganglioside epitope on their surface. With progressing development, neuronal cell labeling is more and more restricted to nerve fibers and especially to cellular adhesion zones, including synaptic terminals, thus suggesting a functional involvement of polysialogangliosides in nerve sprouting and initiation of both cell‐to‐extracellular matrix and cell‐to‐cell contacts.