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Effect of Glucose Starvation on Glucose Transport in Neuronal Cells in Primary Culture from Rat Brain
Author(s) -
Hara Masayuki,
Matsuda Yoshihiro,
Okumura Nobuaki,
Hirai Keisuke,
Nakagawa Hachiro
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb02541.x
Subject(s) - cycloheximide , glucose transporter , starvation , medicine , glucose uptake , endocrinology , biology , transporter , carbohydrate metabolism , cell culture , basal (medicine) , snf3 , biochemistry , protein biosynthesis , insulin , gene , genetics
The regulation of glucose transport into cultured brain cells during glucose starvation was studied. On glucose deprivation for 40 h, 2‐deoxy‐ d ‐glucose (2‐DG) uptake was stimulated twofold in neuronal cells but was not changed significantly in astrocytes. On refeeding, the increased activity of neuronal cells rapidly returned to the basal level, an observation indicating that the effect of glucose starvation was reversible. The increase was due solely to change in the V max , a finding suggesting that the number of glucose transporters on the plasma membrane is increased in starved cells. Cycloheximide inhibited this increase. In the presence of cycloheximide, the activity of 2‐DG uptake of starved cells remained constant for 12 h and then slowly decreased, whereas that of fed cells decreased rapidly. These findings suggest that glucose starvation regulates glucose transport by changing the rate of net synthesis of the transporter in neuronal cells in culture.