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Characteristics of Glucose Transport in Neuronal Cells and Astrocytes from Rat Brain in Primary Culture
Author(s) -
Hara Masayuki,
Matsuda Yoshihiro,
Hirai Keisuke,
Okumura Nobuaki,
Nakagawa Hachiro
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb02540.x
Subject(s) - cytochalasin b , astrocyte , adenosine , neuroglia , biochemistry , biology , forskolin , inosine , cytochalasin , microbiology and biotechnology , cell , cytoskeleton , central nervous system , endocrinology , in vitro
Glucose transport systems in cultured neuronal cells and astrocytes of rats were characterized by measuring the uptake of 2‐deoxy‐ d ‐[ 3 H]glucose ([ 3 H]2‐DG) into the cells. Various sugars inhibited 2‐DG uptake by neuronal cells and astrocytes similarly, a finding indicating that the substrate specificities of the transporters in the two types of cells were almost the same. However, the K m values for 2‐DG of neuronal cells and astrocytes were 1.7 and 0.36 m M , respectively. The uptake of 2‐DG was strongly inhibited by cytochalasin B. Nucleosides, such as adenosine, inosine, and uridine, inhibited 2‐DG uptake competitively in both neuronal cells and astrocytes. The uptakes by both types of cells were also inhibited by forskolin, but not by cyclic AMP, an observation suggesting that forskolin bound directly to the transporters to cause inhibition. Its inhibition was competitive in astrocytes and noncompetitive in neuronal cells. Astrocytes contained a glucose transporter with a subunit molecular weight of 45K, as estimated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis after photoamnity labeling using [ 3 H]cytochalasin B as a probe.