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Concentrations of Tryptoline and Methtryptoline in Rat Brain
Author(s) -
Peura Pekka,
Johnson Jodie V.,
Yost Richard A.,
Faull Kym F.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb02531.x
Subject(s) - homogenization (climate) , chemistry , brain tissue , chromatography , mass spectrometry , gas chromatography–mass spectrometry , cerebellum , cerebral cortex , endocrinology , biology , neuroscience , biodiversity , ecology
Abstract: Combined gas chromatography‐mass spectrometry and gas chromatography‐tandem mass spectrometry have been used to identify and quantify tryptoline, methtryptoline, 5‐hydroxytryptoline, and 5‐hydroxymethtryptoline as their heptafluorobutyryl derivatives in extracts of rat brain. Tryptoline and methtryptohne were identified on the basis of their retention times and mass spectral characteristics: they were reliably detected in brain tissue extracts without interference from artifactual formation; their whole brain concentrations ranged between 0.2 and 3 ng/g; and they had a similar neu‐roanatomical distribution, with the highest concentrations in the cerebellum and the cortex. Smaller quantities of 5‐hydroxytryptoline and 5‐hydroxymethtryptoline were also identified on the basis of their retention times and mass spectral characteristics. However, the significance of this finding is unclear, because these two compounds were accompanied by larger quantities of their tetradeuterated analogues formed from tetradeuterated‐5‐hydroxytryptamine added at the time of tissue homogenization; this result suggests that formation of 5‐hydroxytryptoline and 5‐hydroxymethtryptoline occurred during tissue homogenization, sample preparation, or both.