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Subcellular Distribution of Tyrosine Hydroxylase in Some Catecholaminergic Rat Brain Areas Determined by a Quantitative Immunoblot Assay
Author(s) -
Gillon J. Y.,
Labatut R.,
Renaud B.,
Pujol J. F.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb02508.x
Subject(s) - catecholaminergic , tyrosine hydroxylase , catecholaminergic cell groups , medulla , cell fractionation , biology , free nerve ending , tyrosine , tyrosine 3 monooxygenase , dopaminergic , medulla oblongata , cell bodies , catecholamine , dopamine , neuroscience , chemistry , central nervous system , microbiology and biotechnology , endocrinology , biochemistry , enzyme
The subcellular distribution of the protein tyrosine hydroxylase (TH) after fractionation of rat brain tissue was studied by a sensitive technique of immunoblot quantification in the dopaminergic nigrostriatal and the dorsal noradrenergic pathways and in the ventrolateral medulla. This repartition indicates that in all catecholaminergic regions of the cell bodies studied, the contribution of the nerve endings to the total TH amount is very low (< 7%), in contrast to that observed in the terminal fields. The correlative subcellular determination of the TH amount and activity in the same tissue could be a useful approach for studying experimentally induced mechanisms of catecholamine synthesis modulation in different brain catecholaminergic pathways.