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Effect of Lithium and of Other Drugs Used in the Treatment of Manic Illness on the Cation‐Transporting Properties of Na + , K + ‐ATPase in Mouse Brain Synaptosomes
Author(s) -
Wood A. J.,
Elphick M.,
GrahameSmith D. G.
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb01845.x
Subject(s) - lithium (medication) , chemistry , pharmacology , brain chemistry , medicine , psychiatry
We have developed and used a novel technique to investigate the effects of lithium and other psychotropic drugs on the cation‐transporting properties of the sodium‐ and potassium‐activated ATPase enzyme (Na + , K + ‐ATPase) in intact synaptosomes. Rubidium‐86 uptake into intact synaptosomes is an active process and is inhibited by ∼75% in the presence of the Na + , K + ‐ATPase inhibitor acetylstrophanthidin. In vitro addition of lithium to synaptosomes prepared from untreated mice causes a progressive inhibition of acetylstrophanthidin‐sensitive 86 Rb uptake, but only at concentrations higher than the clinical therapeutic range. However, pretreatment of mice for 14 days in vivo with lithium, carbamazepine, and haloperidol, but not phenytoin, causes a significant stimulation of 86 Rb uptake into synaptosomes via Na + , K + ‐ATPase.