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Nerve Growth Factor Induces Na + , K + ‐ATPase in a Nerve Cell Line
Author(s) -
Inoue Nobuo,
Matsui Hideo,
Hatanaka Hiroshi
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb13254.x
Subject(s) - nerve growth factor , ouabain , endocrinology , medicine , chemistry , cell culture , enzyme , atpase , biology , biochemistry , sodium , receptor , genetics , organic chemistry
The effects of nerve growth factor (NGF) on induction of Na + , K + ‐ATPase were examined in a rat pheochromocytoma cell line, PC12h. Na + , K + ‐ATPase activity in a crude particulate fraction from the cells increased from 0.37 ± 0.02(n = 19) to 0.55 ± 0.02 (n = 20) (means ± SEM, μmol P i /min/mg of protein) when cultured with NGF for 5–11 days. The increase caused by NGF was prevented by addition of specific anti‐NGF antibodies. Epidermal growth factor and insulin had only a small effect on induction of Na + , K + ‐ATPase. A concentration of basic fibroblast growth factor three times higher than that of NGF showed a similar potency to NGF. The molecular form of the enzyme was judged as only the α form in both the untreated and the NGF‐treated cells by a simple pattern of low‐affinity interaction with cardiotonic steroids: inhibition of enzyme activity by strophanthidin ( K i ∼ 1 m M ) and inhibition of Rb + uptake by ouabain ( K i ∼ 100μ M ). As a consequence, during differentiation of PC12h cells to neuron‐like cells, NGF increases the α form of Na + , K + ‐ATPase, but does not induce the α(+) form of the enzyme, which has a high sensitivity for cardiotonic steroid and is a characteristic form in neurons.