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Occurrence of a Large Ca 2+ ‐Independent Release of Glutamate During Anoxia in Isolated Nerve Terminals (Synaptosomes)
Author(s) -
SanchezPrieto Jose,
González Pilar
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb10611.x
Subject(s) - glutamate receptor , chemistry , synaptosome , neuroscience , biophysics , biochemistry , biology , membrane , receptor
Isolated rat cerebral cortical synaptosomes made anoxic by addition of cyanide developed an inhibition of the Ca 2+ ‐dependent release of glutamate 2 min after the addition of the metabolic inhibitor when the intrasynaptosomal ATP/ADP ratio decreased below 1.7. In contrast, cyanide induced a continuous efflux of glutamate through a Ca 2+ ‐independent pathway that accounted for the release of 25% of total intrasynaptosomal glutamate in 5 min. The results suggest that a Ca 2+ ‐independent release of glutamate could be implicated in the neurotoxic action of this amino acid during anoxia.