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Uptake of γ‐Aminobutyric Acid by a Synaptic Vesicle Fraction Isolated from Rat Brain
Author(s) -
Fykse Else M.,
Fonnum Frode
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb10599.x
Subject(s) - synaptic vesicle , nipecotic acid , electrochemical gradient , density gradient , biochemistry , biophysics , chemistry , vesicle , differential centrifugation , atpase , biology , neurotransmitter , membrane , enzyme , receptor , physics , quantum mechanics
γ‐Aminobutyric acid (GABA) was taken up by a MgATP‐dependent mechanism into synaptic vesicles isolated by hypoosmotic shock and density gradient centrifugation. The properties of the vesicular uptake differed clearly from those of synaptosomal and glial uptake, both with respect to Na + , Mg 2+ , and ATP dependence and with respect to response to general GABA uptake inhibitors such as nipecotic acid, diaminobutyric acid, and β‐alanine. The uptake showed a K m of 5.6 m M and a net uptake rate of 1,500 pmol/min/mg of protein. It is suggested that the vesicular uptake of GABA is driven by an electrochemical proton gradient generated by a Mg 2+ ‐ATPase.