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Histamine Metabolites in Cerebrospinal Fluid of the Rhesus Monkey ( Macaca mulatto ): Cisternal‐Lumbar Concentration Gradients
Author(s) -
Prell George D.,
Khandelwal Jai K.,
Burns R. Stanley,
Green Jack Peter
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb10592.x
Subject(s) - cerebrospinal fluid , metabolite , histamine , medicine , endocrinology , histaminergic , lumbar , chemistry , biology , anatomy
Similar to metabolites of other aminergic transmitters, histamine metabolites of brain, tele ‐methylhistamine (t‐MH) and tele ‐methylimidazoleacetic acid (t‐MIAA), could have a concentration gradient between rostral and caudal sites of CSF. To test this hypothesis, cisternal and lumbar CSF samples were collected in pairs from eight monkeys ( Macaca mulatta ), and levels of t‐MH and t‐MIAA were measured by gas chromatography‐mass spectrometry. pros ‐Methylimidazoleacetic acid (p‐MIAA), an endogenous isomer of t‐MIAA that is not a histamine metabolite, was also measured. Cisternal levels (in picomoles per milliliter, mean ± SEM) of t‐MH (9.9 ± 1.4) and t‐MIAA (40.8 ± 7.6), but not of p‐MIAA (9.7 ± 1.2), exceeded those in lumbar CSF (t‐MH, 1.8 ± 0.3; t‐MIAA, 6.8 ± 0.9; p‐MIAA, 8.6 ± 0.6) in every monkey. The magnitudes of the mean cisternal‐lumbar concentration gradients fort‐MH(6.6 ± 1.1)and t‐MIAA (6.5 ± 1.3) were indistinguishable. These gradients exceed those of metabolites of most other transmitters. There was no gradient for the levels of p‐MIAA. The cisternal, but not lumbar, levels of t‐MH and t‐MIAA were correlated. There was no significant difference between the means of the metabolite concentration ratios (t‐MIAAJ.t‐MH) in cisternal (4.0 ± 0.4) and lumbar (4.4 ± 0.9) CSF. The steepness of these gradients suggests that levels of t‐MH and t‐MIAA in lumbar CSF might be useful probes of histaminergic metabolism in brain.