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Different Subcellular Localization of Neurotensin‐Receptor and Neurotensin‐Acceptor Sites in the Rat Brain Dopaminergic System
Author(s) -
Schotte Alain,
Rostène William,
Laduron Pierre M.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb10568.x
Subject(s) - olfactory tubercle , neurotensin , nucleus accumbens , caudate nucleus , dopaminergic , medicine , putamen , striatum , endocrinology , neurotensin receptor , biology , dopamine , chemistry , receptor , neuropeptide , biochemistry
The subcellular localization of neurotensin‐receptor sites (NT 2 sites) and neurotensin‐acceptor sites (NT 1 sites) was studied in rat caudate‐putamen by isopycnic centrifugation in sucrose density gradients. [ 3 H]Neurotensin binding to NT 2 sites occurred as a major peak at higher sucrose densities, colocalized with [ 3 H]dopamine uptake, and as a small peak at a lower density; whereas binding to NT, sites occurred as a single large peak at an intermediate density. 6‐Hydroxydopamine lesions of the median forebrain bundle resulted in a total loss of NT 2 sites in the caudate‐putamen but did not affect NT 2 sites in the nucleus accumbens and the olfactory tubercle. NT 1 sites were not affected. Kainic acid injections into the rat caudate‐putamen led to a partial decrease of NT, sites in this region 5 days later. After a few weeks they returned to normal. Therefore NT 2 sites are probably associated with presynaptic nigrostriatal dopaminergic terminals in the caudate‐putamen but not in the nucleus accumbens and the olfactory tubercle. A possible association of NT 1 sites with glial cells is suggested.