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γ‐Aminobutyric Acid Agonist‐Induced Alterations in the Ultrastructure of Cultured Cerebellar Granule Cells Is Restricted to Early Development
Author(s) -
Hansen Gert H.,
Belhage Bo,
Schousboe Arne,
Meier Eddi
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb04862.x
Subject(s) - granule (geology) , golgi apparatus , ultrastructure , endoplasmic reticulum , organelle , microbiology and biotechnology , cytoplasm , cerebellum , chemistry , vesicle , biology , biochemistry , endocrinology , anatomy , paleontology , membrane
Abstract The effect of 4,5,6,7‐tetrahydroisoxazolo[5,4‐ c ]‐pyridin‐3‐ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 μ M ) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7‐day‐old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14‐day‐old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.