Premium
Relationship Between the Nerve Growth Factor‐Regulated Clone 73 Gene Product and the 58‐Kilodalton Neuronal Intermediate Filament Protein (Peripherin)
Author(s) -
Aletta John M.,
Angeletti Ruth,
Liem Ronald K. H.,
Purcell Clement,
Shelanski Michael L.,
Greene Lloyd A.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb03104.x
Subject(s) - peripherin , protein filament , nerve growth factor , kilodalton , biology , neurofilament , intermediate filament protein , intermediate filament , clone (java method) , microbiology and biotechnology , gene product , messenger rna , gene , biochemistry , gene expression , cell , cytoskeleton , receptor , immunohistochemistry , immunology
Exposure of PC12 cells to nerve growth factor (NGF) has been shown to induce an rnRNA that encodes a novel neuronal intermediate filament protein. The findings presented here concern the identity of this filament protein. The major protein in NGF‐treated PC12 cell cytoskeletons derived by extraction with 1% Triton X‐100 is of apparent M r = 58,000, focuses by isoelectric focusing as several closely spaced spots of pl 5.6–5.8, and is elevated relative to non‐NGF‐treated cells. Partial microsequencing of this material reveals 2 internal sequences that are identical to a 14‐residue sequence encoded by the NGF‐regulated clone 73 mRNA, but not to sequences of other known proteins. An antiserum raised against a 19‐residue synthetic peptide corresponding to the deduced C‐terminus of the protein encoded by the NGF‐regulated clone 73 mRNA specifically recognizes the 58,000‐M r protein. Properties of the 58‐kilodalton protein strongly suggest that it corresponds to an intermediate filament protein (peripherin) previously identified in PC12 cells and in peripheral and certain CNS neurons. Identification of the intermediate filament protein encoded by an NGF‐induced message should facilitate studies of its regulation and function.