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Induction of Rat Brain Tubulin Following Ammonium Ingestion
Author(s) -
Felipo Vicente,
Miñana MariaDolores,
Azorín Inmaculada,
Grisolía Santiago
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb03065.x
Subject(s) - tubulin , ingestion , ammonium acetate , ammonium , protein biosynthesis , oral administration , chemistry , medicine , endocrinology , biochemistry , biology , pharmacology , chromatography , high performance liquid chromatography , microtubule , microbiology and biotechnology , organic chemistry
The effect of oral administration of ammonium acetate for 2, 15, 30, and 100 days on protein synthesis in rat brain was investigated. Although protein synthesis changes were modest, i.e., maximal increase of 24%, there was induction of synthesis and accumulation of a protein with an M r of 55,000. We show, on the basis of its position on two‐dimensional electrophoresis and its immunological reactivity, that this protein is tubulin. Its content increased by 33% as determined by isolation of tubulin after 15 days of oral administration of ammonium and to 49% after 100 days as determined by quantitative immunoblotting.