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Prostaglandin Induces Ca 2+ Influx and Cyclic GMP Formation in Mouse Neuroblastoma × Rat Glioma Hybrid NG108–15 Cells in Culture
Author(s) -
Miwa Naoto,
Sugino Hiroaki,
Ueno Ryuji,
Hayaishi Osamu
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb03025.x
Subject(s) - extracellular , intracellular , verapamil , chemistry , medicine , endocrinology , nifedipine , cytosol , cell culture , calcium , biochemistry , biology , enzyme , genetics
Various prostaglandins (PGs) (10 n M ‐30 μ M ) were added to NG108–15 cells in culture, and changes in the levels of intracellular cyclic GMP and Ca 2+ were investigated. Exposure of the cells to PGF 2α , PGD 2 , and PGE 2 (10 μ M ) transiently increased the cyclic GMP content 7.5‐, 3.9‐, and 3.1‐fold, respectively. Furthermore, the increased levels of cyclic GMP correlated well with the rise in cytosolic free Ca 2+ concentrations induced by the PGs. Other PGs (10 μ M ), including metabolites and synthetic analogs, which had no effect on intracellular Ca 2+ , failed to increase the cyclic GMP content in the cells. When extracellular Ca 2+ was depleted from the culture medium, the PG‐induced increase in cyclic GMP level was almost completely abolished. In addition, treatment of the cells with quin 2 tetraacetoxymethyl ester dose‐dependently inhibited the PG‐induced cyclic GMP formation. The increase in cyclic GMP content caused by treatment of the cells with a high K + level (50 m M ) was completely blocked by voltage‐dependent Ca 2+ entry blockers, such as verapamil (10 μ M ), nifedipine (1 μ M ), and diltiazem (100 μ M); however, the PG (10 μ M )‐induced increase in cyclic GMP content was not affected by such Ca 2+ entry blockers. These findings indicate that PG‐induced cyclic GMP formation may require the rise in intracellular Ca 2+ level and that the voltage‐dependent Ca 2+ channels may not be involved in the PG‐induced rise in Ca 2+ content.