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Depletion of Brain Glutathione in Preweanling Mice by L‐Buthionine Sulfoximine
Author(s) -
Slivka Adam,
Spina Mary Beth,
Calvin Harold I.,
Cohen Gerald
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb03021.x
Subject(s) - glutathione , buthionine sulfoximine , spinal cord , endocrinology , medicine , central nervous system , biology , chemistry , neuroscience , biochemistry , enzyme
Previous studies indicated that DL‐buthionine sulfoximine (dl‐BSO), an agent that inhibits the biosynthesis of GSH in liver and other peripheral organs, fails to suppress levels of GSH in the CNS. In the current study, preweanling mice responded to repeated injections of l‐BSO with marked declines (79.6–86.5%) of GSH content in brain and spinal cord. In adult mice, the same treatment schedule produced only modest declines (17.8–29.2%) of GSH content in brain and a 55.9% decline in spinal cord. Pretreatment of preweanling mice with L‐BSO represents a tool for studying the role of GSH in the CNS.