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K + ‐Dependent Stimulation of Dopamine Synthesis in Striatal Synaptosomes Is Mediated by Protein Kinase C
Author(s) -
Chowdhury Muhammad,
Fillenz Marianne
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb02956.x
Subject(s) - dopamine , stimulation , neuroscience , synaptosome , chemistry , protein kinase a , biology , kinase , biochemistry , central nervous system
Dopamine synthesis rate was measured in striatal synaptosomes. Removal of Na + increased synthesis rate; this was blocked in Ca 2+ ‐free medium and by addition of the Ca 2+ /calmodulin inhibitor N ‐6‐aminohexyl‐5‐chloro‐1‐naphthalenesulfonamide (W7). The increase in dopamine synthesis rate caused by the addition of the phorbol ester 12‐ O ‐tetradecanoylphorboI‐13‐acetate (TPA) was blocked by the protein kinase C inhibitor polymyxin B. K + ‐stimulated synthesis was unchanged in Ca 2+ ‐free medium or by addition of W7; it was blocked by polymyxin B. The effect of 50 m M K + was additive with that of 8‐Br cyclic AMP and of Na + removal; the combined effect of 50 m M K + and TPA was no greater than that of either alone. These results suggest that stimulation of dopamine synthesis in striatal synaptosomes by 50 m M K + is mediated by protein kinase C.