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Expression of Myelin Components in Mouse Schwann Cells in Culture
Author(s) -
Burroni D.,
White F. V.,
Ceccarini C.,
Matthieu J.M.,
CostantinoCeccarin E.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb02917.x
Subject(s) - galactocerebroside , myelin , immunoprecipitation , schwann cell , biology , in vitro , microbiology and biotechnology , neurite , cell culture , biochemistry , neuroscience , genetics , central nervous system , oligodendrocyte
Mouse Schwann cells cultured in vitro are capable of expressing basal levels of the major myelin components P 1 , P 2, P 0, and galactocerebroside. Numerical counts of immunostained cultures indicated that between 22 and 40% of the cells are positive up to 21 days for all of the components indicated. Electrophoretic analysis of Schwann cells labeled with a 14 C‐amino acid mixture revealed the presence of proteins with relative mobilities identical to those of P 0 and P 1 . Positive identification of the two proteins was indicated by immunoprecipitation of P 1 and immunoblotting of P 0 . These data show that in the absence of neurites, Schwann cells in culture can express low levels of myelin characteristic components even in the absence of myelin assembly.