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Developmental Changes in the Molecular Weights of Polypeptides in the Human CNS That Carry the HNK‐1 Epitope and Bind Phaseolus vulgaris Lectins
Author(s) -
Mikol Daniel D.,
Wrabetz Larry,
Marton Linda S.,
Stefansson Kari
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb02498.x
Subject(s) - epitope , phaseolus , biochemistry , biology , lectin , glycoprotein , monoclonal antibody , antibody , genetics , botany
The binding patterns of electrophoresed polypeptides from homogenates of human frontal lobe, cerebellum, and spinal cord obtained at various stages of development were determined for several lectins with specificities for a wide range of oligosaccharides. A discrete developmental change in the molecular‐weight pattern was seen only among polypeptides binding the two Phaseolus vulgaris agglutinins, E‐phytohemagglutinin (E‐PHA) and L‐PHA. With increasing maturity, the apparent molecular weights of the major polypeptides binding these two lectins progressively decreased. Furthermore, at all stages of development, E‐PHA and L‐PHA bound to the same polypeptides as the monoclonal antibody HNK‐1, which recognizes a carbohydrate epitope on polypeptides that may play roles in cell adhesion. Based on the carbohydrate specificities of the two PHAs, we conclude that it is likely that the HNK‐1 epitope resides on a triantennary N‐linked oligosaccharide bisected by N ‐acetylglucosamine.