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Increase in Extracellular Dopamine in the Striatum During Cerebral Ischemia: A Study Utilizing Cerebral Microdialysis
Author(s) -
Slivka Actam,
Brannan Timothy S.,
Weinberger Jesse,
Knott Peter J.,
Cohen Gerald
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb02468.x
Subject(s) - microdialysis , dopamine , homovanillic acid , extracellular , striatum , in vivo , ischemia , medicine , chemistry , endocrinology , extracellular fluid , 3,4 dihydroxyphenylacetic acid , biology , biochemistry , serotonin , receptor , microbiology and biotechnology
Unilateral ligation of the left common carotid artery in anesthetized Mongolian gerbils resulted in a steep rise in extracellular dopamine in the ipsilateral striatum in 9 out of 19 animals. Extracellular dopamine was measured by cerebral dialysis in vivo and reached a peak of 0.19 m M at 40 min. At the same time, the level of homovanillic acid fell, whereas the levels of ascorbate and 3,4‐dihydroxyphenylacetic acid remained relatively constant. In a separate group of animals studied with a combined dialysis/electrochemistry probe, a rise in the in vivo chronoamperometric signal in three out of six animals correlated with a rise in extracellular dopamine. The number of animals responding in these experiments (roughly 50%) corresponds to the frequency of incompetent Circle of Willis, as well as literature reports of the frequency of signs of stroke in unanesthetized gerbils. These results show a remarkable accumulation of dopamine in extracellular fluid in response to cerebral ischemia. Released dopamine appears to be responsible for the elevated in vivo electrochemical signal previously reported.