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Neurite Extension and Increased Expression of R 1 Cyclic AMP‐Binding Protein in Ouabain‐Resistant Neuroblastoma Mutants
Author(s) -
Meyer Sharon A.,
Lin Zheng,
Liu Alice Y.C.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01832.x
Subject(s) - neurite , neuroblastoma , cell culture , cellular differentiation , ouabain , intracellular , biology , mutant , phenotype , microbiology and biotechnology , in vitro , chemistry , biochemistry , genetics , sodium , gene , organic chemistry
Morphological and biochemical parameters of neuroblastoma differentiation were assessed in 12 clonal derivatives of the N‐18 mouse neuroblastoma cell line selected for their ouabain‐resistant ( oua r ) property. When cultured in a normal growth medium, nine of the 12 oua r cell lines exhibited a more complex pattern of neurite outgrowth than the parental N‐18 cells. The morphological pattern most frequently observed with the oua r cells was the extension of several branched processes per cell. This pattern of spontaneous neurite outgrowth in the o u a r cell lines can be correlated with an increase in expression of the 47,000‐dalton R 1 cyclic AMP (cAMP)‐binding protein. The growth rate, intracellular level of cAMP, and acetylcholin‐esterase activity of the oua r cell lines were not significantly different from those of the parental N‐18 neuroblastoma cells. Treatment of the parental and oua r neuroblastoma cell lines with 1 m M N 6 , O 2 ‐dibutyryl cAMP promoted an elaborate pattern of neurite outgrowth and marked increases in acetylcholinesterase and R 1 cAMP‐binding activities. The distinctive pattern of differentiation phenotype exhibited by the oua r cells and the dibutyryl cAMP‐induced differentiated neuroblastoma cells suggests that these two protocols yielded different degrees of differentiation. Furthermore, our results suggest a linkage of the biochemical events underlying ouabain resistance and expression of differentiation phenotypes in the mouse neuroblastoma cells.