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Distribution of Thiamine, Thiamine Phosphates, and Thiamine Metabolizing Enzymes in Neuronal and Glial Cell Enriched Fractions of Rat Brain
Author(s) -
Laforenza Umberto,
Patrini Cesare,
Rindi Gianguido
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01805.x
Subject(s) - thiamine , enzyme , biochemistry , chemistry , distribution (mathematics) , mathematics , mathematical analysis
The distribution of thiamine, thiamine phos‐phoesters, and the thiamine pyrophosphate synthetizing [thiamine‐pyrophosphokinase (TPKase)] as well as hydro‐lyzing [thiamine pyrophosphatase (TPPase) and thiamine monophosphatase (TMPase)] enzymes was determined in neuronal and glial enriched fractions prepared from rat brain. Nucleoside diphosphatases [inosine diphosphatase (IDPase) and uridine diphosphatase (UDPase)] and nucleoside monophosphatases [uridine monophosphatase (UMPase) and inosine monophosphatase (IMPase)] were also determined. Thiamine and thiamine mono‐ and pyrophosphate were present in neuronal enriched fractions at concentrations 2.8, 3.6, and 4.6 times higher than in glial fractions. TMPase was found only in glial enriched fractions, whereas the levels of TPKase, UMPase, IMPase, IDPase, UDPase, and TPPase were 2.0‐, 2.2‐, 1.3‐, 2.8‐, 3.7‐, and 20.8‐fold higher in neuronal than in glial fractions.