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Glucose Transporter of the Blood‐Brain Barrier and Brain in Chronic Hyperglycemia
Author(s) -
Harik Sami I.,
Gravina Stephen A.,
Kalaria Rajesh N.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01180.x
Subject(s) - endocrinology , medicine , streptozotocin , blood–brain barrier , glucose transporter , cytochalasin b , polyuria , polydipsia , phlorizin , cerebral cortex , diabetes mellitus , chemistry , central nervous system , insulin , biochemistry , in vitro
Abstract: The effect of chronic hyperglycemia on the glucose transporter moiety of the blood‐brain barrier and cerebral cortex was studied in rats 3 weeks after the administration of a single intravenous dose of streptozotocin (60 mg/kg), using specific [ 3 H]cytochalasin B binding methods. Streptozotocin‐treated rats developed hyperglycemia, as well as polydipsia and polyuria, and failed to gain weight. The density of d‐glucose‐displaceable cytochalasin B binding sites in the brain microvessels of streptozotocin‐treated hyperglycemic rats was increased by about 30% compared with those of control rats, without change in the affinity of binding. Chronic hyperglycemia had no effect on the density or affinity of specific binding of cytochalasin B to cerebral cortical membranes. These findings do not support the hypothesis that glucose transporters in brain microvessels comprising the blood‐brain barrier are “down‐regulated” in chronic hyperglycemia.