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Effects of Extracellular Potassium on Glycogen Stores of Astrocytes In Vitro
Author(s) -
CambrayDeakin Martin,
Pearce Brian,
Morrow Christine,
Murphy Sean
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01167.x
Subject(s) - glycogen , potassium , astrocyte , extracellular , endocrinology , chemistry , medicine , cerebral cortex , biology , biochemistry , central nervous system , organic chemistry
Astrocyte‐enriched and meningeal cell cultures of the rat cerebral cortex were prepared, and their glycogen content was measured after 10–90 min under control (2.5 m M ) concentrations of potassium after prefeeding with 20 m M glucose. No net change in glycogen level was noted in either culture over this period. Cell cultures were then exposed to increased concentrations of potassium (5, 10, and 15 m M ), and their glycogen content was measured after 10–90 min. Both types of cell culture showed complex and variable changes in glycogen content. In general, increased potassium concentrations caused astrocyte glycogen stores to be reduced at physiological increases of potassium levels (from 2.5 to 5 m M and above), although a period of resynthesis was evident at all potassium concentrations. Meningeal cell glycogen levels were highly variable and only affected by high (10 and 15 m M ) levels of potassium. These results are discussed with respect to the theory that changes in the external potassium concentration caused by neuronal activity might act as a signal controlling astrocyte glycogen stores.