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Conservation of Amino Acid Sequences Between Human and Porcine Choline Acetyltransferase
Author(s) -
Hersh Louis B.,
Takane Karen,
Gylys Karen,
Moomaw Carolyn,
Slaughter Clive
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01166.x
Subject(s) - choline acetyltransferase , choline , amino acid , biochemistry , acetyltransferase , chemistry , biology , computational biology , neuroscience , cholinergic , gene , acetylation
The amino acid sequence of 11 peptides generated from human placental choline acetyltransferase was compared to the corresponding amino acid sequences predicted from the nucleotide sequence of a recently cloned porcine choline acetyltransferase cDNA. These peptides, which were generated by cyanogen bromide cleavage or tryptic digestion, accounted for 23% of the amino acids in the enzyme. Of the 145 amino acids sequenced eight differed between the two species, yielding an identity of 94% over the regions sampled. Of the eight amino acids that differed six could represent single base changes in the DNA sequence. These findings demonstrate strong sequence similarity between porcine and human choline acetyltransferase and indicate that they are closely related evolutionarily.