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Isatin: Identity with the Purified Endogenous Monoamine Oxidase Inhibitor Tribulin
Author(s) -
Glover Vivette,
Halket J. M.,
Watkins P. J.,
Clow Angela,
Goodwin B. L.,
Sandier M.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01089.x
Subject(s) - isatin , monoamine oxidase , monoamine oxidase inhibitor , chemistry , endogeny , monoamine oxidase a , biochemistry , monoamine oxidase b , pharmacology , chromatography , biology , enzyme , organic chemistry
Purified tribulin, an endogenous monoamine oxidase (MAO) inhibitor, has been identified by direct probe insertion mass spectrometry as the indole‐2,3‐dione, isatin. A gas chromatographic‐mass spectrometric assay for isatin has been developed and used to measure its relatively high concentrations in unpurified human urine, and in rat heart and brain. Isatin is a known compound with a broad range of biological activity; this is the first report of its presence in the animal body. Isatin is a potent inhibitor of MAO, particularly of MAO B (IC 50 , 3 μ M ), and also binds to central benzodiazepine receptors (IC 50 against clonazepam, 123 μ M ).