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Effect of Increased Glucose Levels on Na + /K + ‐Pump Activity in Cultured Neuroblastoma Cells
Author(s) -
Yorek Mark A.,
Dunlap Joyce A.,
Ginsberg Barry H.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01081.x
Subject(s) - inositol , neuroblastoma , sorbinil , sorbitol , endocrinology , medicine , fructose , metabolism , carbohydrate metabolism , intracellular , aldose reductase inhibitor , extracellular , chemistry , aldose reductase , ouabain , biology , cell culture , biochemistry , sodium , diabetes mellitus , receptor , organic chemistry , genetics
Neuroblastoma cells were used to analyze the effect of elevated glucose levels on myo ‐inositol metabolism and Na + /K + ‐pump activity. The activity of the Na + /K + pump in neuroblastoma cells is almost totally sensitive to ouabain inhibition. Culturing neuroblastoma cells in 30 m M glucose caused a significant decrease in Na + /K + ‐pump activity, myo ‐inositol metabolism, and myo‐inositol content, compared to cells grown in the presence of 30 m M fructose. Glucose supplementation also caused a large intracellular accumulation of sorbitol. The aldose reductase inhibitor sorbinil prevented the abnormalities in myo‐ inositol metabolism and partially restored Na + /K + ‐pump activity in neuroblastoma cells cultured in the presence of elevated glucose levels. These results suggest that the accumulation of sorbitol by neuroblastoma cells exposed to elevated concentrations of extracellular glucose causes a decrease in myo ‐inositol metabolism and these abnormalities are associated with a reduction in Na + /K + ‐pump activity.