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Formation of γ‐Amino‐β‐Hydroxybutyric Acid from 2‐Hydroxyputrescine in Rat Brain
Author(s) -
Noto Tadashi,
Hasegawa Takeshi,
Nakao Junji,
Kamimura Hiroshi,
Harada Hiroyuki,
Nakajima Teruo
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01073.x
Subject(s) - neuroscience , chemistry , psychology , biochemistry
γ‐Amino‐β‐[ 3 H]hydroxybutyric acid ([ 3 H]‐GABOB) was formed in rat brain from 2‐[ 3 H]‐hydroxyputrescine that had been chemically synthesized from 2‐oxoputrescine and [ 3 H]sodium borohydride. After the injection of 2‐[ 3 H]hydroxyputrescine into the lateral ventricle of a rat brain, the rat was killed and then the brain was removed. [ 3 H]GABOB in the brain was identified by a combination of ion‐exchange chromatography, high‐voltage paper electrophoresis, and recrystallization of the radioactive compound with authentic GABOB.