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Chaotropic Ions Affect the Conformation of Quisqualate Receptors in Rat Cortical Membranes
Author(s) -
Honoré Tage,
Drejer Jørgen
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01060.x
Subject(s) - chaotropic agent , receptor , affect (linguistics) , membrane , neuroscience , biophysics , chemistry , microbiology and biotechnology , biology , psychology , biochemistry , communication
Binding of [ 3 H]( R,S )‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid ([ 3 H]AMPA) to quisqualate receptors in the presence of SCN ions produced curvilinear Scatchard plots. Kinetic investigations of [ 3 H]‐AMPA binding showed that the curvilinearity cannot be explained by assuming binding to two separate binding sites or by considering it due to cooperative interaction. A more likely explanation is that the quisqualate receptors exist in two states, one with high and one with low affinity for [ 3 H]AMPA. Chaotropic ions change the relaxation constant between the two states.