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Identification as Synapsin of a Synaptosomal Protein Immunoreacting with Anti‐Myelin Basic Protein Antiserum
Author(s) -
Pedraza Liliana T.,
Roth German A.,
Cumar Federico A.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01054.x
Subject(s) - myelin , synapsin i , myelin basic protein , antiserum , major basic protein , gel electrophoresis , biochemistry , polyacrylamide gel electrophoresis , proteolipid protein 1 , microbiology and biotechnology , chemistry , biology , synapsin , antibody , immunology , synaptic vesicle , central nervous system , enzyme , vesicle , neuroscience , membrane , eosinophil , asthma
Rat brain proteins able to react with anti‐myelin basic protein antiserum, raised under conditions to induce experimental allergic encephalomyelitis in rabbits, were examined by immunoblot methods after sodium dodecyl sul‐fate‐polyacrylamide gel electrophoresis. Apart from the four forms of myelin basic protein present in rat brain, the antiserum detected other proteins of higher molecular weight. Subcellular fractionation shows that these high‐molecular‐weight proteins are relatively concentrated in a syn‐aptosome‐enriched fraction compared to a myelin fraction. A major protein fraction immunorelated to myelin basic protein migrated in the gels as a doublet with apparent molecular weights of approximately 80K and 86K; these proteins were tentatively identified as synapsin la and Ib. A purified synapsin preparation analyzed by immunoblot after two‐dimensional gel electrophoresis also reacted with anti‐myelin basic protein antisera. When the serum was purified by affinity chromatography on a myelin basic protein‐conjugated Sepharose column the nonadsorbed material lost this activity whereas the eluted antibodies reacted with myelin basic protein and synapsin. In addition, sequence amino acid comparison of decapeptides showed some homology between these two proteins. A possible implication of immunological agents against myelin basic protein cross‐reacting with extra‐myelin proteins in the process of experimental allergic encephalomyelitis is considered.