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Biosynthesis of Angiotensinogen and Angiotensins by Brain Cells in Primary Culture
Author(s) -
Hermann Klaus,
Phillips M. Ian,
Hilgenfeldt Ulrich,
Raizada Mohan K.
Publication year - 1988
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1988.tb01052.x
Subject(s) - primary culture , primary (astronomy) , biosynthesis , renin–angiotensin system , endocrinology , neuroscience , medicine , cell culture , psychology , biology , biochemistry , enzyme , genetics , physics , blood pressure , astronomy
This study focuses on the ability of primary rat brain cells in culture to synthesize angiotensinogen, angio‐tensin I, and angiotensin II. HPLC in combination with radioimmunoassay was used to characterize these compounds. Following incubation with 3 H‐labeled isoleucine, radioactively labeled angiotensinogen with an approximate molecular weight of 25,000 was identified in both glial and neuronal cells. Other molecular weight forms of angiotensinogen with molecular weights of about 300 and 160,000 were present in both cell types. In addition to angiotensinogen, radioactively labeled angiotensin I and angiotensin II were also synthesized by neuronal and glial cells. These results suggest that glial and neuronal cells can synthesize angiotensinogen, angiotensin I, and angiotensin II in a similar manner shown for the peripheral renin angiotensin system.