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γ‐Aminobutyric Acid‐Activated 36 Cl ‐ Influx: A Functional In Vitro Assay for CNS γ‐Aminobutyric Acid Receptors of Insects
Author(s) -
Wafford K. A.,
Sattelle D. B.,
Abalis I.,
Eldefrawi A. T.,
Eldefrawi M. E.
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb13144.x
Subject(s) - picrotoxin , periplaneta , bicuculline , aminobutyric acid , receptor , stimulation , gamma aminobutyric acid , in vitro , gabaa receptor , chemistry , cockroach , biochemistry , biophysics , biology , endocrinology , ecology
The effects of γaminobutyric acid (GABA) on the uptake of 36 Cl into a membrane microsac preparation from isolated nerve cords of the cockroach Periplaneta americana was studied. On addition of 1 μ M GABA (after 4‐s incubation, then rapid quenching) the influx of 36 Cl ‐ was stimulated to a level 75% above that of the control value. This stimulation was reduced by picrotoxin (100 μ M ), but was not significantly affected by bicuculline (100 μ M )Results of 36 Cl ‐ influx experiments are in agreement with data obtained from radiolabelled ligand binding assays and electrophysiological investigations on the same tissue. The method described represents a functional in vitro assay for CNS GABA receptors of insects.