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Insulin Enhances Development of Functional Voltage‐Dependent Ca 2+ Channels in Aneurally Cultured Human Muscle
Author(s) -
Desnuelle Claude,
Askanas Valerie,
Engel W. King
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb10003.x
Subject(s) - endocrinology , antagonist , medicine , growth factor , epidermal growth factor , receptor , biology , insulin , voltage dependent calcium channel , binding site , chemistry , calcium , biochemistry
Voltage‐dependent Ca 2+ channels were studied by the binding of the potent Ca 2+ channel antagonist PN200‐MO and by the K + ‐induced 45 Ca 2+ uptake in human muscle cultured aneurally in the presence of insulin, fibroblast growth factor, and epidermal growth factor, added in combination or individually. Compared to the muscle grown in medium without growth factors, 14–15 days of treatment with insulin (10 μg/rnI) alone or in combination with two other growth factors caused a 3.4‐and 3.8‐fold increase per culture dish in the number of PN200–110 binding sites, respectively. There was no change in the affinity of the ligand‐receptor complex. Under the same conditions, there was also a fourfold increase of the K + ‐in‐duced 45 Ca 2+ uptake in cultured human muscle. Neither fibroblast growth factor nor epidermal growth facto alone influenced PN200–110 binding sites. Our study demonstrates that insulin enhances the development of functional voltage‐dependent Ca 2+ channels in cultured human muscle.