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Regional Distribution of High‐Affinity γ‐[ 3 H] Hydroxybutyrate Binding Sites as Determined by Quantitative Autoradiography
Author(s) -
Hechler Viviane,
Weissmann Dinah,
March Evelyne,
Pujol JeanFrançois,
Maitre Michel
Publication year - 1987
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1987.tb09990.x
Subject(s) - cerebellum , pons , entorhinal cortex , hippocampus , biology , diencephalon , limbic system , thalamus , endocrinology , medicine , binding site , central nervous system , cortex (anatomy) , biochemistry , anatomy , neuroscience
The distribution of high‐affinity binding sites for γ‐[3H]hydroxybutyrate in coronal sections of rat brain was studied by quantitative autoradiographic techniques. Binding sites for this naturally occumng substance, which may possibly have a neurotransmitter role, are concentrated in some restricted areas of the brain, particularly in the limbic system. The hippocampus (especially field CAI of Ammon's horn, at 292 fmol/mg of tissue), septum (72 fmol/mg of tissue), and cortex (frontal, 113 fmol/mg of tissue; parietal, 103 fmol/mg of tissue; cingulate, 114 fmol/mg of tissue; and entorhinal, 134 fmol/mg of tissue) show pronounced labeling with γ‐[ 3 H]hydroxybutyrate. Binding is much lower in caudatus‐putamen (50 fmol/mg of tissue), thalamus, and hypothalamus. Caudal parts of the brain (cerebellum, pons, and medulla) are practically devoid of binding sites. These results strongly support a functional role of endogenous y‐hydroxybutyrate in particularly restricted areas of the rat brain.